cck 8 working solution  (Dojindo Labs)

Journal: Bioactive Materials

Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation

doi: 10.1016/j.bioactmat.2026.04.003

Figure Lengend Snippet: In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: On days 1, 3, and 5 of culture, the existing culture medium was substituted with 100 μL of CCK-8 working solution (Cell Counting Kit-8, Beyotime Biotech, China), prepared by mixing fresh α-MEM and CCK-8 reagent at a volume ratio of 10:1.

Techniques: In Vitro, CCK-8 Assay, Cell Culture, Staining, Migration

Journal: Frontiers in Pharmacology

Article Title: Rus-GXF, a ruscogenin glycoside, binds to the ADP-binding domain of JAK1 to prevent inflammation and barrier damage in acute lung injury

doi: 10.3389/fphar.2026.1730503

Figure Lengend Snippet: Anti-inflammatory effect of monomeric Rus-GXF on LPS-induced RAW264.7 cells. (A) CCK-8 assay of Rus-GXF on RAW264.7 cells ( n = 6). (B–D) Cell culture supernatant NO, IL-6 and TNF-α content assay ( n = 4). (E,F) Real-time fluorescence quantitative PCR assay to detect the relative gene expression of COX2 and iNOS ( n = 4). Bars are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: After 6 h, cells were treated with serially diluted Rus-GXF (RAW264.7: 0, 1, 10, 20, 40, 60 μM; BEAS-2B: 0, 1, 10, 20, 40, 80, 160 μM; n = 3) for 24 h. Then, 110 μL of CCK-8 working solution (CCK-8/complete medium = 1/10; Beijing Solarbio Science & Technology Co., Ltd.) was added per well.

Techniques: CCK-8 Assay, Cell Culture, Fluorescence, Real-time Polymerase Chain Reaction, Gene Expression

Journal: Frontiers in Pharmacology

Article Title: Rus-GXF, a ruscogenin glycoside, binds to the ADP-binding domain of JAK1 to prevent inflammation and barrier damage in acute lung injury

doi: 10.3389/fphar.2026.1730503

Figure Lengend Snippet: Modulation of inflammatory responses and barrier function by Rus-GXF in BEAS-2B cells. (A) CCK-8 assay of Rus-GXF on BEAS-2B cells ( n = 6). (B) Real-time fluorescence quantitative PCR assay to detect the relative gene expression of IL-6 and TNF-α ( n = 4). (C) The gene expression of CXCL1 and CXCL2. (D) The gene expression of ZO-1 and Occludin ( n = 4). Bars are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: After 6 h, cells were treated with serially diluted Rus-GXF (RAW264.7: 0, 1, 10, 20, 40, 60 μM; BEAS-2B: 0, 1, 10, 20, 40, 80, 160 μM; n = 3) for 24 h. Then, 110 μL of CCK-8 working solution (CCK-8/complete medium = 1/10; Beijing Solarbio Science & Technology Co., Ltd.) was added per well.

Techniques: CCK-8 Assay, Fluorescence, Real-time Polymerase Chain Reaction, Gene Expression